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e coli jm109 cells  (TaKaRa)


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    Structured Review

    TaKaRa e coli jm109 cells
    E Coli Jm109 Cells, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 345 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e+coli+jm109+cells/Monoclonal+Anti-mouse+P-cadherin/pm41518045-21-13-17
    Average 95 stars, based on 345 article reviews
    e coli jm109 cells - by Bioz Stars, 2026-09
    95/100 stars

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    Transformation Assay:

    Article Title: Aedes aegypti aminopeptidase N3 is a functional binding receptor of Bacillus thuringiensis subsp. israelensis Cry4Ba toxin
    Article Snippet: The resulting PCR products were then cloned into the pCold-GST vector using the In-Fusion Snap Assembly Master Mix (Takara, Japan) according to the manufacturer’s instructions. .. Subsequently, assembly product was transformed into E. coli JM109 cells (Takara, Japan). .. For protein expression, the pColdAeAPN3 recombinant plasmid was further transfected into E. coli BL21 (DE3) cells (Takara, Japan).

    Article Title: Development of an Effective DNA Vaccine Against Viral Edema of Carp/Koi Sleepy Disease Caused by Carp Edema Virus.
    Article Snippet: Since viral edema of carp (VEC) or koi sleepy disease (KSD), in koi carp (Cyprinus carpio) caused by carp edema virus (CEV) infection was first reported in Japan (Hosoya and Suzuki 1976; Murakami et al. 1976; Sugai and Koike 1980; Ono et al. 1986; Suzuki and Fukuda 1987), it has led to substantial economic losses in cultured koi and common carp worldwide (Way et al. 2017; Machat et al. 2021).. The infection manifests fusion of secondary gill filaments and clubbed gills, resulting in impaired respiration and osmoregulation (Ono et al. 1986; Oyamatsu, Hata, et al. 1997; Oyamatsu, Matoyama, et al. 1997; Miyazaki et al. 2005; Pikula et al. 2021; Matras et al. 2023).. CEV belongs to the family Poxviridae.

    Article Title: Scattered migrating colony formation in the filamentous cyanobacterium, Pseudanabaena sp. NIES-4403
    Article Snippet: .. The amplified DNA fragment was further cloned into the pGEM-T Easy vector (Promega, USA) and transformed into E. coli JM109 cells (Takara Bio, Japan). .. The PCR-derived segments of the resulting plasmids were sequenced (Applied Biosystems 3730xl, Thermo Fisher, USA) using the same primers, and the resulting sequence was compared to 16S rRNA gene sequences from 29 cyanobacterial species (downloaded from the Ribosomal Database Project (RDP) on Mar 23, 2018) [ ].

    Plasmid Preparation:

    Article Title: Molecular cloning and characterization of the two putative toxins expressed in the venom of the devil stinger Inimicus japonicus.
    Article Snippet: Oligonucleotides were purchased from Sigma-Aldrich., and the NucleoSpin RNA II kit from Takara (Otsu, Japan). .. Plasmid vector pTAC2 was obtained from the BioDynamics Laboratory (Tokyo, Japan), plasmid vectors pET-19 b and E. coli BL21 (DE3)pLysS from Merck (Darmstadt, Germany), and E. coli JM109 cells and the In-Fusion HD Cloning Kit were purchased from Takara (Otsu, Japan). .. The chromogenic substrate for PLA2, 4-nitro-3-(octanoyloxy)benzoic acid (NOB), was obtained from Enzo Life Sciences (Farmingdale, USA), and all other chemicals were of analytical grade for biochemical use.

    Article Title: Scattered migrating colony formation in the filamentous cyanobacterium, Pseudanabaena sp. NIES-4403
    Article Snippet: .. The amplified DNA fragment was further cloned into the pGEM-T Easy vector (Promega, USA) and transformed into E. coli JM109 cells (Takara Bio, Japan). .. The PCR-derived segments of the resulting plasmids were sequenced (Applied Biosystems 3730xl, Thermo Fisher, USA) using the same primers, and the resulting sequence was compared to 16S rRNA gene sequences from 29 cyanobacterial species (downloaded from the Ribosomal Database Project (RDP) on Mar 23, 2018) [ ].

    Cloning:

    Article Title: Molecular cloning and characterization of the two putative toxins expressed in the venom of the devil stinger Inimicus japonicus.
    Article Snippet: Oligonucleotides were purchased from Sigma-Aldrich., and the NucleoSpin RNA II kit from Takara (Otsu, Japan). .. Plasmid vector pTAC2 was obtained from the BioDynamics Laboratory (Tokyo, Japan), plasmid vectors pET-19 b and E. coli BL21 (DE3)pLysS from Merck (Darmstadt, Germany), and E. coli JM109 cells and the In-Fusion HD Cloning Kit were purchased from Takara (Otsu, Japan). .. The chromogenic substrate for PLA2, 4-nitro-3-(octanoyloxy)benzoic acid (NOB), was obtained from Enzo Life Sciences (Farmingdale, USA), and all other chemicals were of analytical grade for biochemical use.

    Article Title: Novel Ca 2+ ‐independent carbohydrate recognition of the C‐type lectins, SPL‐1 and SPL‐2, from the bivalve Saxidomus purpuratus
    Article Snippet: Plasmid vector pET‐3a and E. coli BL21(DE3)pLysS were obtained from Novagen. .. E. coli JM109 cells, SMARTer cDNA Cloning Kit, and In‐Fusion HD Cloning Kit were obtained from Clontech. ..

    other:

    Article Title: Scattered migrating colony formation in the filamentous cyanobacterium, Pseudanabaena sp. NIES-4403
    Article Snippet: Cells were cultured on BG-11 medium [49] containing 1.5 % of Bacto TM agar (BD Falcon, 362 USA) under continuous light illuminated by fluorescent lamps (around 30 μmol m −2 s −1 ) at 30 °C.

    Amplification:

    Article Title: Scattered migrating colony formation in the filamentous cyanobacterium, Pseudanabaena sp. NIES-4403
    Article Snippet: .. The amplified DNA fragment was further cloned into the pGEM-T Easy vector (Promega, USA) and transformed into E. coli JM109 cells (Takara Bio, Japan). .. The PCR-derived segments of the resulting plasmids were sequenced (Applied Biosystems 3730xl, Thermo Fisher, USA) using the same primers, and the resulting sequence was compared to 16S rRNA gene sequences from 29 cyanobacterial species (downloaded from the Ribosomal Database Project (RDP) on Mar 23, 2018) [ ].

    Clone Assay:

    Article Title: Scattered migrating colony formation in the filamentous cyanobacterium, Pseudanabaena sp. NIES-4403
    Article Snippet: .. The amplified DNA fragment was further cloned into the pGEM-T Easy vector (Promega, USA) and transformed into E. coli JM109 cells (Takara Bio, Japan). .. The PCR-derived segments of the resulting plasmids were sequenced (Applied Biosystems 3730xl, Thermo Fisher, USA) using the same primers, and the resulting sequence was compared to 16S rRNA gene sequences from 29 cyanobacterial species (downloaded from the Ribosomal Database Project (RDP) on Mar 23, 2018) [ ].



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